› 2hh0

X-ray diffraction
2.85Å resolution

Structure of an Anti-PrP Fab, P-Clone, in Complex with its Cognate Bovine Peptide Epitope.

Released:
DOI: 10.2210/pdb2hh0/pdb
PDB entry 2hh0 coloured by chain, front view.
PDB entry 2hh0 coloured by chain, side view.
PDB entry 2hh0 coloured by chain, top view.
Source organisms:
Primary publication:
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation.
Luginbühl B, Kanyo Z, Jones RM, Fletterick RJ, Prusiner SB, Cohen FE, Williamson RA, Burton DR, Plückthun A
J Mol Biol 363 75-97 (2006)
PMID: 16962610

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210462 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Light Chain, P-Clone Fab, Chimera Chain: L
Molecule details ›
Chain: L
Length: 210 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Heavy Chain, P-Clone Fab, Chimera Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Major prion protein Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P10279 (Residues: 107-115; Coverage: 4%)
Gene names: PRNP, PRP

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3121
Unit cell:
a: 119.834Å b: 119.834Å c: 95.534Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.222 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 review citations

Therapeutic antibodies: successes, limitations and hopes for the future.
Chames et al. (2009)
12 more

3 mentions without citation

Crystal structure of human prion protein bound to a therapeutic antibody.
Antonyuk et al. (2009)
2 more