› 2h00

X-ray diffraction
2Å resolution

Human methyltransferase 10 domain containing protein

Released:
DOI: 10.2210/pdb2h00/pdb
PDB entry 2h00 coloured by chain, front view.
PDB entry 2h00 coloured by chain, side view.
PDB entry 2h00 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Min JR, Wu H, Zeng H, Loppnau P, Weigelt J, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + adenine in U6 snRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in U6 snRNA
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA N6-adenosine-methyltransferase METTL16 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 254 amino acids
Theoretical weight: 28.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86W50 (Residues: 40-291; Coverage: 45%)
Gene names: METT10D, METTL16
Sequence domains: RNA methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

3 bound ligands:
Ligand SAH 3 x SAH
Ligand DTU 1 x DTU
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P3121
Unit cell:
a: 133.737Å b: 133.737Å c: 78.833Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.221 0.274
Expression system: Escherichia coli

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