› 2grx

X-ray diffraction
3.3Å resolution

Crystal structure of TonB in complex with FhuA, E. coli outer membrane receptor for ferrichrome

Released:
DOI: 10.2210/pdb2grx/pdb
PDB entry 2grx coloured by chain, front view.
PDB entry 2grx coloured by chain, side view.
PDB entry 2grx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of TonB in complex with FhuA, E. coli outer membrane receptor.
Pawelek PD, Croteau N, Ng-Thow-Hing C, Khursigara CM, Moiseeva N, Allaire M, Coulton JW
Science 312 1399-402 (2006)
PMID: 16741125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136389 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Ferrichrome outer membrane transporter/phage receptor Chains: A, B
Molecule details ›
Chains: A, B
Length: 725 amino acids
Theoretical weight: 80.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06971 (Residues: 34-438, 439-747; Coverage: 100%)
Gene names: JW0146, b0150, fhuA, tonA
Sequence domains:
Structure domains:
Protein TonB Chains: C, D
Molecule details ›
Chains: C, D
Length: 229 amino acids
Theoretical weight: 24.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02929 (Residues: 29-239; Coverage: 88%)
Gene names: JW5195, b1252, exbA, tonB
Sequence domains:
Structure domains: TonB

Ligands and Environments

Carbohydrate polymer : NEW Components: GCN, KDO, GMH
Carbohydrate polymer
7 bound ligands:
Ligand PO4 2 x PO4
Ligand FTT 8 x FTT
Ligand DPO 2 x DPO
Ligand DAO 2 x DAO
Ligand EAP 2 x EAP
Ligand FCI 2 x FCI
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 234.32Å b: 91.84Å c: 138.51Å
α: 90° β: 118.86° γ: 90°
R-values:
R R work R free
0.297 0.284 0.329
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
38 more

21 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
20 more