› 2gln

X-ray diffraction
1.98Å resolution

Crystal structure of Mycobacterium tuberculosis trHbN, GlnE11Ala mutant

Released:
DOI: 10.2210/pdb2gln/pdb
PDB entry 2gln coloured by chain, front view.
PDB entry 2gln coloured by chain, side view.
PDB entry 2gln coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Ligand interactions in the distal heme pocket of Mycobacterium tuberculosis truncated hemoglobin N: roles of TyrB10 and GlnE11 residues.
Ouellet Y, Milani M, Couture M, Bolognesi M, Guertin M
Biochemistry 45 8770-81 (2006)
PMID: 16846220

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 1 truncated hemoglobin GlbN Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 14.41 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN25 (Residues: 1-136; Coverage: 100%)
Gene names: MTCY48.23, Rv1542c, glbN
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

4 bound ligands:
Ligand NA 1 x NA
Ligand PO4 2 x PO4
Ligand CYN 2 x CYN
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 45.777Å b: 60.443Å c: 87.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.173 0.233
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Mycobacterial truncated hemoglobins: from genes to functions.
Ascenzi et al. (2007)
2 more

2 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
1 more