› 2gaf

X-ray diffraction
2.4Å resolution

Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)

Released:
DOI: 10.2210/pdb2gaf/pdb
PDB entry 2gaf coloured by chain, front view.
PDB entry 2gaf coloured by chain, side view.
PDB entry 2gaf coloured by chain, top view.
Source organism: Vaccinia virus
Primary publication:
Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity.
Moure CM, Bowman BR, Gershon PD, Quiocho FA
Mol Cell 22 339-49 (2006)
PMID: 16678106

Function and Biology Details

Reactions catalysed: 
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149942 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 297 amino acids
Theoretical weight: 35.06 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1PIV4 (Residues: 1-297; Coverage: 89%)
Gene names: VACAC2_106, VACCL3_106, VACV-DUKE-103, VACV_091, VACV_TT12_115, VAC_DPP10_106, VAC_DPP12_106, VAC_DPP13_106, VAC_DPP15_106, VAC_DPP16_106, VAC_DPP17_106, VAC_DPP19_106, VAC_DPP20_106, VAC_DPP21_106, VAC_DPP9_106, VACli_106, synVACV_106
Sequence domains: Poly A polymerase regulatory subunit
Structure domains: Vaccinia Virus protein VP39
Poly(A) polymerase catalytic subunit Chain: D
Molecule details ›
Chain: D
Length: 469 amino acids
Theoretical weight: 54.39 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P23371 (Residues: 11-479; Coverage: 98%)
Gene names: E1L, OPG063, PAPL, VACWR057
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 69.085Å b: 91.26Å c: 133.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

4 review citations

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
3 more

7 mentions without citation

Poxvirus proteomics and virus-host protein interactions.
Van Vliet et al. (2009)
6 more