› 2fw1

X-ray diffraction
2Å resolution

Structure of PurE (N5-carboxyaminoimidazole ribonucleotide mutase) from the acidophilic bacterium Acetobacter aceti, at pH 8.5

Released:
DOI: 10.2210/pdb2fw1/pdb
PDB entry 2fw1 coloured by chain, front view.
PDB entry 2fw1 coloured by chain, side view.
PDB entry 2fw1 coloured by chain, top view.
Source organism: Acetobacter aceti
Primary publication:
Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti.
Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, Francois JA, Goodman RA, Kappock TJ
Biochemistry 45 8193-208 (2006)
PMID: 16819818

Function and Biology Details

Reaction catalysed: 
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo octamer (preferred)
homo hexadecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-173882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N5-carboxyaminoimidazole ribonucleotide mutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 18.88 KDa
Source organism: Acetobacter aceti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2QJL3 (Residues: 1-182; Coverage: 100%)
Gene name: purE
Sequence domains: AIR carboxylase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I422
Unit cell:
a: 99.43Å b: 99.43Å c: 164.168Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.184
Expression system: Escherichia coli

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Citations

3 review citations

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
2 more