› 2fun

X-ray diffraction
3Å resolution

alternative p35-caspase-8 complex

Released:
DOI: 10.2210/pdb2fun/pdb
PDB entry 2fun coloured by chain, front view.
PDB entry 2fun coloured by chain, side view.
PDB entry 2fun coloured by chain, top view.
Source organisms:
Primary publication:
Native chemical ligation in covalent caspase inhibition by p35.
Lu M, Min T, Eliezer D, Wu H
Chem Biol 13 117-22 (2006)
PMID: 16492559

Function and Biology Details

Reaction catalysed: 
Strict requirement for Asp at position P1 and has a preferred cleavage sequence of (Leu/Asp/Val)-Glu-Thr-Asp-|-(Gly/Ser/Ala)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139942 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Early 35 kDa protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 298 amino acids
Theoretical weight: 34.74 KDa
Source organism: Autographa californica nucleopolyhedrovirus
Expression system: Escherichia coli
UniProt:
  • Canonical: P08160 (Residues: 2-299; Coverage: 100%)
Gene name: P35
Sequence domains: Apoptosis preventing protein
Structure domains: Baculovirus p35
Caspase-8 subunit p18 Chains: B, D
Molecule details ›
Chains: B, D
Length: 258 amino acids
Theoretical weight: 29.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14790 (Residues: 222-479; Coverage: 54%)
Gene names: CASP8, MCH5
Sequence domains: Caspase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: C2221
Unit cell:
a: 99.97Å b: 117.34Å c: 346.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.229 0.26
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Viral subversion of apoptotic enzymes: escape from death row.
Best SM. (2008)
4 more

3 mentions without citation

Small Molecule Active Site Directed Tools for Studying Human Caspases.
Poreba et al. (2015)
2 more