› 2ftx

X-ray diffraction
1.9Å resolution

Crystal structure of the yeast kinetochore Spc24/Spc25 globular domain

Released:
DOI: 10.2210/pdb2ftx/pdb
PDB entry 2ftx coloured by chain, front view.
PDB entry 2ftx coloured by chain, side view.
PDB entry 2ftx coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Entry authors: Wei RR, Harrison SC

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154001 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinetochore protein SPC25 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40014 (Residues: 133-221; Coverage: 40%)
Gene names: SPC25, YER018C
Structure domains: Chromosome segregation protein Spc25
Kinetochore protein SPC24 Chain: B
Molecule details ›
Chain: B
Length: 64 amino acids
Theoretical weight: 7.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04477 (Residues: 154-213; Coverage: 28%)
Gene names: SPC24, YM9718.16C, YMR117C
Sequence domains: Spc24 subunit of Ndc80
Structure domains: Double Stranded RNA Binding Domain

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I4122
Unit cell:
a: 84.907Å b: 84.907Å c: 93.195Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.226
Expression system: Escherichia coli

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