› 2fqo

X-ray diffraction
1.87Å resolution

Crystal structure of B. subtilis LuxS in complex with (2S)-2-Amino-4-[(2R,3R)-2,3-dihydroxy-3-N- hydroxycarbamoyl-propylmercapto]butyric acid

Released:
DOI: 10.2210/pdb2fqo/pdb
PDB entry 2fqo coloured by chain, front view.
PDB entry 2fqo coloured by chain, side view.
PDB entry 2fqo coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Design and synthesis of substrate and intermediate analogue inhibitors of S-ribosylhomocysteinase.
Shen G, Rajan R, Zhu J, Bell CE, Pei D
J Med Chem 49 3003-11 (2006)
PMID: 16686542

Function and Biology Details

Reaction catalysed: 
S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128577 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-ribosylhomocysteine lyase Chain: A
Molecule details ›
Chain: A
Length: 157 amino acids
Theoretical weight: 17.74 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34667 (Residues: 1-157; Coverage: 100%)
Gene names: BSU30670, luxS, ytjB
Sequence domains: S-Ribosylhomocysteinase (LuxS)
Structure domains: S-ribosylhomocysteinase (LuxS)

Ligands and Environments

3 bound ligands:
Ligand CO 1 x CO
Ligand SO4 1 x SO4
Ligand HYI 1 x HYI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P6522
Unit cell:
a: 62.81Å b: 62.81Å c: 148.64Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

19 review citations

Exploiting quorum sensing to confuse bacterial pathogens.
LaSarre et al. (2013)
18 more

3 mentions without citation

Structural insights into the acyl intermediates of the Plasmodium falciparum fatty acid synthesis pathway: the mechanism of expansion of the acyl carrier protein core.
Upadhyay et al. (2009)
2 more