› 2flo

X-ray diffraction
2.2Å resolution

Crystal structure of exopolyphosphatase (PPX) from E. coli O157:H7

Released:
DOI: 10.2210/pdb2flo/pdb
PDB entry 2flo coloured by chain, front view.
PDB entry 2flo coloured by chain, side view.
PDB entry 2flo coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Primary publication:
The structure of the exopolyphosphatase (PPX) from Escherichia coli O157:H7 suggests a binding mode for long polyphosphate chains.
Rangarajan ES, Nadeau G, Li Y, Wagner J, Hung MN, Schrag JD, Cygler M, Matte A
J Mol Biol 359 1249-60 (2006)
PMID: 16678853

Function and Biology Details

Reaction catalysed: 
(Polyphosphate)(n) + H(2)O = (polyphosphate)(n-1) + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exopolyphosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 524 amino acids
Theoretical weight: 60.21 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFL8 (Residues: 2-513; Coverage: 100%)
Gene names: ECs3364, Z3765, ppx
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 68 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C, NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 95.547Å b: 132.139Å c: 107.85Å
α: 90° β: 105.1° γ: 90°
R-values:
R R work R free
0.205 0.202 0.247
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Inorganic polyphosphate: essential for growth and survival.
Rao et al. (2009)
4 more

5 mentions without citation

Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference.
Beloglazova et al. (2011)
4 more