› 2fgz

X-ray diffraction
1.75Å resolution

Crystal Structure Analysis of apo pullulanase from Klebsiella pneumoniae

Released:
DOI: 10.2210/pdb2fgz/pdb
PDB entry 2fgz coloured by chain, front view.
PDB entry 2fgz coloured by chain, side view.
PDB entry 2fgz coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Crystal structure of pullulanase: evidence for parallel binding of oligosaccharides in the active site.
Mikami B, Iwamoto H, Malle D, Yoon HJ, Demirkan-Sarikaya E, Mezaki Y, Katsuya Y
J Mol Biol 359 690-707 (2006)
PMID: 16650854

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197832 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: C2
Unit cell:
a: 148.984Å b: 60.214Å c: 132.791Å
α: 90° β: 112.612° γ: 90°
R-values:
R R work R free
0.183 0.183 0.214

Quick links

Citations

10 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
9 more

4 mentions without citation

Group B streptococcus pullulanase crystal structures in the context of a novel strategy for vaccine development.
Gourlay et al. (2009)
3 more