› 2fb3

X-ray diffraction
2.35Å resolution

Structure of MoaA in complex with 5'-GTP

Released:
DOI: 10.2210/pdb2fb3/pdb
PDB entry 2fb3 coloured by chain, front view.
PDB entry 2fb3 coloured by chain, side view.
PDB entry 2fb3 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Binding of 5'-GTP to the C-terminal FeS cluster of the radical S-adenosylmethionine enzyme MoaA provides insights into its mechanism.
Hänzelmann P, Schindelin H
Proc Natl Acad Sci U S A 103 6829-34 (2006)
PMID: 16632608

Function and Biology Details

Reaction catalysed: 
GTP + S-adenosyl-L-methionine + reduced electron acceptor = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159674 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP 3',8-cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 340 amino acids
Theoretical weight: 39.13 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P69848 (Residues: 1-340; Coverage: 100%)
Gene names: SAOUHSC_02536, moaA
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

6 bound ligands:
Ligand SO4 2 x SO4
Ligand MET 2 x MET
Ligand SF4 4 x SF4
Ligand GTP 1 x GTP
Ligand 5AD 1 x 5AD
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 48.015Å b: 103.449Å c: 191.217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.22 0.271
Expression system: Escherichia coli

Quick links

Citations

30 review citations

The Radical SAM Superfamily.
Frey et al. (2008)
29 more

26 mentions without citation

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
25 more