› 2f95

X-ray diffraction
2.2Å resolution

M intermediate structure of sensory rhodopsin II/transducer complex in combination with the ground state structure

Released:
DOI: 10.2210/pdb2f95/pdb
PDB entry 2f95 coloured by chain, ensemble of 2 models, front view.
PDB entry 2f95 coloured by chain, ensemble of 2 models, side view.
PDB entry 2f95 coloured by chain, ensemble of 2 models, top view.
Source organism: Natronomonas pharaonis
Primary publication:
Development of the signal in sensory rhodopsin and its transfer to the cognate transducer.
Moukhametzianov R, Klare JP, Efremov R, Baeken C, Göppner A, Labahn J, Engelhard M, Büldt G, Gordeliy VI
Nature 440 115-9 (2006)
PMID: 16452929

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154503 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sensory rhodopsin-2 Chain: A
Molecule details ›
Chain: A
Length: 248 amino acids
Theoretical weight: 26.53 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42196 (Residues: 2-239; Coverage: 100%)
Gene names: sop2, sopII
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins
Sensory rhodopsin II transducer Chain: B
Molecule details ›
Chain: B
Length: 163 amino acids
Theoretical weight: 17.22 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42259 (Residues: 5-157; Coverage: 29%)
Gene names: htr2, htrII
Sequence domains:
Structure domains: Helix hairpin bin

Ligands and Environments

2 bound ligands:
Ligand BOG 1 x BOG
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 124.62Å b: 46.9Å c: 53.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
18 more

7 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
6 more