› 2f47

X-ray diffraction
1.7Å resolution

Xray crystal structure of T4 lysozyme mutant L20/R63A liganded to methylguanidinium

Released:
DOI: 10.2210/pdb2f47/pdb
PDB entry 2f47 coloured by chain, front view.
PDB entry 2f47 coloured by chain, side view.
PDB entry 2f47 coloured by chain, top view.
Source organism: Tequatrovirus T4
Primary publication:
Guanidinium derivatives bind preferentially and trigger long-distance conformational changes in an engineered T4 lysozyme.
Yousef MS, Bischoff N, Dyer CM, Baase WA, Matthews BW
Protein Sci 15 853-61 (2006)
PMID: 16600969

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.69 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-39, 40-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand MGX 1 x MGX
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3221
Unit cell:
a: 60.685Å b: 60.685Å c: 95.482Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.206 0.217
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Studies on the functional significance of a C-terminal S-shaped motif in human arginase type I: essentiality for cooperative effects.
García et al. (2009)
1 more

1 mention without citation

Coarse-grained description of protein internal dynamics: an optimal strategy for decomposing proteins in rigid subunits.
Potestio et al. (2009)