› 2f3x

X-ray diffraction
3.1Å resolution

Crystal structure of FapR (in complex with effector)- a global regulator of fatty acid biosynthesis in B. subtilis

Released:
DOI: 10.2210/pdb2f3x/pdb
PDB entry 2f3x coloured by chain, front view.
PDB entry 2f3x coloured by chain, side view.
PDB entry 2f3x coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural basis of lipid biosynthesis regulation in Gram-positive bacteria.
Schujman GE, Guerin M, Buschiazzo A, Schaeffer F, Llarrull LI, Reh G, Vila AJ, Alzari PM, de Mendoza D
EMBO J 25 4074-83 (2006)
PMID: 16932747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor FapR Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 17.75 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34835 (Residues: 42-188; Coverage: 78%)
Gene names: BSU15880, fapR, ylpC
Sequence domains: Thioesterase superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

1 bound ligand:
Ligand MLC 2 x MLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 89.441Å b: 89.441Å c: 162.166Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.226
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Membrane lipid homeostasis in bacteria.
Zhang et al. (2008)
17 more

5 mentions without citation

Structural basis of lipid biosynthesis regulation in Gram-positive bacteria.
Schujman et al. (2006)
4 more