› 2ewv

X-ray diffraction
2.8Å resolution

Crystal Structure of the Pilus Retraction Motor PilT and Bound ADP

Released:
DOI: 10.2210/pdb2ewv/pdb
PDB entry 2ewv coloured by chain, front view.
PDB entry 2ewv coloured by chain, side view.
PDB entry 2ewv coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.
Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT
Structure 15 363-76 (2007)
PMID: 17355871

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AAA+ ATPase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 372 amino acids
Theoretical weight: 42.09 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O66950 (Residues: 1-366; Coverage: 100%)
Gene names: aq_745, pilT
Sequence domains: Type II/IV secretion system protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: P6
Unit cell:
a: 108.24Å b: 108.24Å c: 69.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.267
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
23 more

5 mentions without citation

Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.
Satyshur et al. (2007)
4 more