› 2e5w

X-ray diffraction
2Å resolution

Crystal structure of spermidine synthase from Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2e5w/pdb
PDB entry 2e5w coloured by chain, front view.
PDB entry 2e5w coloured by chain, side view.
PDB entry 2e5w coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Mizutani H, Kunishima N, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129643 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine aminopropyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 32.01 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57950 (Residues: 1-280; Coverage: 100%)
Gene names: PH0211, speE
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ACT 4 x ACT
Ligand AG3 2 x AG3
Ligand MTA 2 x MTA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2
Unit cell:
a: 149.411Å b: 122.735Å c: 102.085Å
α: 90° β: 117.69° γ: 90°
R-values:
R R work R free
0.195 0.194 0.215
Expression system: Escherichia coli

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