› 2e4g

X-ray diffraction
2.08Å resolution

RebH with bound L-Trp

Released:
DOI: 10.2210/pdb2e4g/pdb
PDB entry 2e4g coloured by chain, front view.
PDB entry 2e4g coloured by chain, side view.
PDB entry 2e4g coloured by chain, top view.
Source organism: Lentzea aerocolonigenes
Primary publication:
Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases.
Yeh E, Blasiak LC, Koglin A, Drennan CL, Walsh CT
Biochemistry 46 1284-92 (2007)
PMID: 17260957

Function and Biology Details

Reaction catalysed: 
Tryptophan + FADH(2) + chloride + O(2) + H(+) = 7-chloro-L-tryptophan + FAD + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan 7-halogenase RebH Chains: A, B
Molecule details ›
Chains: A, B
Length: 550 amino acids
Theoretical weight: 62.54 KDa
Source organism: Lentzea aerocolonigenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KHZ8 (Residues: 1-530; Coverage: 100%)
Gene names: rbmJ, rebH
Sequence domains: Tryptophan halogenase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments

1 bound ligand:
Ligand TRP 2 x TRP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P62
Unit cell:
a: 114.75Å b: 114.75Å c: 231.233Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

Flavin dependent monooxygenases.
Huijbers et al. (2014)
30 more

7 mentions without citation

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
6 more