› 2dt5

X-ray diffraction
2.16Å resolution

Crystal Structure of TTHA1657 (AT-rich DNA-binding protein) from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2dt5/pdb
PDB entry 2dt5 coloured by chain, front view.
PDB entry 2dt5 coloured by chain, side view.
PDB entry 2dt5 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of TTHA1657 (AT-rich DNA-binding protein; p25) from Thermus thermophilus HB8 at 2.16 A resolution.
Nakamura A, Sosa A, Komori H, Kita A, Miki K
Proteins 66 755-9 (2007)
PMID: 17154156

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-177895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Redox-sensing transcriptional repressor Rex Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.18 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SHS3 (Residues: 1-211; Coverage: 100%)
Gene names: TTHA1657, rex
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 6 x SO4
Ligand CL 1 x CL
Ligand NAD 2 x NAD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P43212
Unit cell:
a: 135.595Å b: 135.595Å c: 68.424Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

10 citation in other articles

NADP+ reduction with reduced ferredoxin and NADP+ reduction with NADH are coupled via an electron-bifurcating enzyme complex in Clostridium kluyveri.
Wang et al. (2010)
9 more

4 mentions without citation

How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes.
Lee et al. (2021)
3 more