› 2dd9

X-ray diffraction
2.3Å resolution

A mutant of GFP-like protein from Chiridius poppei

Released:
DOI: 10.2210/pdb2dd9/pdb
PDB entry 2dd9 coloured by chain, front view.
PDB entry 2dd9 coloured by chain, side view.
PDB entry 2dd9 coloured by chain, top view.
Source organism: Chiridius poppei
Primary publication:
Structural basis for red-shifted emission of a GFP-like protein from the marine copepod Chiridius poppei.
Suto K, Masuda H, Takenaka Y, Tsuji FI, Mizuno H
Genes Cells 14 727-37 (2009)
PMID: 19469881

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173729 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 216 amino acids
Theoretical weight: 24.58 KDa
Source organism: Chiridius poppei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2MHN7 (Residues: 2-219; Coverage: 100%)
Gene name: cpGFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

2 bound ligands:
Ligand CXS 5 x CXS
Ligand CL 1 x CL
1 modified residue:
Ligand CR2 4 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 108.75Å b: 113.99Å c: 133.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.218
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Generation of brilliant green fluorescent petunia plants by using a new and potent fluorescent protein transgene.
Chin et al. (2018)
5 more