› 2cw7

X-ray diffraction
2.7Å resolution

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homing endonuclease PI-PkoII Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 62.28 KDa
Source organism: Thermococcus kodakarensis KOD1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77933 (Residues: 852-1388; Coverage: 32%)
Gene names: TK0001, pol
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2221
Unit cell:
a: 107.7Å b: 149.06Å c: 146.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Nature's recipe for splitting inteins.
Aranko et al. (2014)
1 more

7 mentions without citation

Structural and dynamical features of inteins and implications on protein splicing.
Eryilmaz et al. (2014)
6 more