› 2cny

X-ray diffraction
1.9Å resolution

Salmonella enterica SafA pilin in complex with a 19-residue SafA Nte peptide (I15A mutant)

Released:
DOI: 10.2210/pdb2cny/pdb
PDB entry 2cny coloured by chain, front view.
PDB entry 2cny coloured by chain, side view.
PDB entry 2cny coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism.
Remaut H, Rose RJ, Hannan TJ, Hultgren SJ, Radford SE, Ashcroft AE, Waksman G
Mol Cell 22 831-842 (2006)
PMID: 16793551

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187381 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Saf-pilin pilus formation protein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.14 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ZRK4 (Residues: 47-170; Coverage: 86%)
Gene names: STM0299, safA
Sequence domains: Saf-pilin pilus formation protein
Structure domains: Dr adhesin
Saf-pilin pilus formation protein domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 20 amino acids
Theoretical weight: 2.13 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Not provided
UniProt:
  • Canonical: Q8ZRK4 (Residues: 27-46; Coverage: 14%)
Gene names: STM0299, safA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 69.159Å b: 69.159Å c: 126.682Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.212
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

Secretion systems in Gram-negative bacteria: structural and mechanistic insights.
Costa et al. (2015)
29 more

1 mention without citation

The invasin D protein from Yersinia pseudotuberculosis selectively binds the Fab region of host antibodies and affects colonization of the intestine.
Sadana et al. (2018)