› 2cj4

X-ray diffraction
1.63Å resolution

Crystal Structure of a Cell Wall Invertase Inhibitor from Tobacco at pH 4.6

Released:
DOI: 10.2210/pdb2cj4/pdb
PDB entry 2cj4 coloured by chain, front view.
PDB entry 2cj4 coloured by chain, side view.
PDB entry 2cj4 coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range.
Hothorn M, Scheffzek K
Acta Crystallogr D Biol Crystallogr 62 665-70 (2006)
PMID: 16699194

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectinesterase inhibitor domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.03 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli
UniProt:
  • Canonical: O49908 (Residues: 20-166; Coverage: 100%)
Gene name: LOC107808322
Sequence domains: Plant invertase/pectin methylesterase inhibitor
Structure domains: Invertase/pectin methylesterase inhibitor family protein

Ligands and Environments

2 bound ligands:
Ligand ACT 4 x ACT
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21212
Unit cell:
a: 131.35Å b: 47.18Å c: 57.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.151 0.189
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural insights into the pH-controlled targeting of plant cell-wall invertase by a specific inhibitor protein.
Hothorn et al. (2010)
1 more