› 2cde

X-ray diffraction
3.5Å resolution

Structure and binding kinetics of three different human CD1d-alpha- Galactosylceramide specific T cell receptors - iNKT-TCR

Released:
DOI: 10.2210/pdb2cde/pdb
PDB entry 2cde coloured by chain, front view.
PDB entry 2cde coloured by chain, side view.
PDB entry 2cde coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and binding kinetics of three different human CD1d-alpha-galactosylceramide-specific T cell receptors.
Gadola SD, Koch M, Marles-Wright J, Lissin NM, Shepherd D, Matulis G, Harlos K, Villiger PM, Stuart DI, Jakobsen BK, Cerundolo V, Jones EY
J Exp Med 203 699-710 (2006)
PMID: 16520393

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208607 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
INKT-TCR Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 192 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 244 amino acids
Theoretical weight: 27.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 289.36Å b: 84.95Å c: 78.86Å
α: 90° β: 103.3° γ: 90°
R-values:
R R work R free
0.283 0.283 0.35
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

32 review citations

The biology of NKT cells.
Bendelac et al. (2007)
31 more

5 mentions without citation

Crystal structures of mouse CD1d-iGb3 complex and its cognate Valpha14 T cell receptor suggest a model for dual recognition of foreign and self glycolipids.
Zajonc et al. (2008)
4 more