› 2c7g

X-ray diffraction
1.8Å resolution

FprA from Mycobacterium tuberculosis: His57Gln mutant

Released:
DOI: 10.2210/pdb2c7g/pdb
PDB entry 2c7g coloured by chain, front view.
PDB entry 2c7g coloured by chain, side view.
PDB entry 2c7g coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Role of the His57-Glu214 ionic couple located in the active site of Mycobacterium tuberculosis FprA.
Pennati A, Razeto A, de Rosa M, Pandini V, Vanoni MA, Mattevi A, Coda A, Aliverti A, Zanetti G
Biochemistry 45 8712-20 (2006)
PMID: 16846214

Function and Biology Details

Reaction catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH-ferredoxin reductase FprA Chain: A
Molecule details ›
Chain: A
Length: 456 amino acids
Theoretical weight: 49.39 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WIQ3 (Residues: 1-456; Coverage: 100%)
Gene names: MTCY164.16, Rv3106, fprA
Sequence domains: Pyridine nucleotide-disulphide oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand ODP 1 x ODP
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 146.824Å b: 81.04Å c: 41.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Structural and functional diversity of ferredoxin-NADP(+) reductases.
Aliverti et al. (2008)
2 more

1 mention without citation

A unified statistical model to support local sequence order independent similarity searching for ligand-binding sites and its application to genome-based drug discovery.
Xie et al. (2009)