› 2c7b

X-ray diffraction
2.3Å resolution

The Crystal Structure of EstE1, a New Thermophilic and Thermostable Carboxylesterase Cloned from a Metagenomic Library

Released:
DOI: 10.2210/pdb2c7b/pdb
PDB entry 2c7b coloured by chain, front view.
PDB entry 2c7b coloured by chain, side view.
PDB entry 2c7b coloured by chain, top view.
Source organism: uncultured archaeon
Primary publication:
Crystal structure of hyperthermophilic esterase EstE1 and the relationship between its dimerization and thermostability properties.
Byun JS, Rhee JK, Kim ND, Yoon J, Kim DU, Koh E, Oh JW, Cho HS
BMC Struct Biol 7 47 (2007)
PMID: 17625021

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha/beta hydrolase fold-3 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 311 amino acids
Theoretical weight: 34.06 KDa
Source organism: uncultured archaeon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5G935 (Residues: 1-311; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P41212
Unit cell:
a: 73.71Å b: 73.71Å c: 234.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Metagenomic gene discovery: how far have we moved into novel sequence space?
Tuffin et al. (2009)
1 more

18 mentions without citation

Carboxylic ester hydrolases from hyperthermophiles.
Levisson et al. (2009)
17 more