› 2c6y

X-ray diffraction
2.4Å resolution

Crystal structure of interleukin enhancer-binding factor 1 bound to DNA

Released:
DOI: 10.2210/pdb2c6y/pdb
PDB entry 2c6y coloured by chain, front view.
PDB entry 2c6y coloured by chain, side view.
PDB entry 2c6y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human FOXK1a-DNA complex and its implications on the diverse binding specificity of winged helix/forkhead proteins.
Tsai KL, Huang CY, Chang CH, Sun YJ, Chuang WJ, Hsiao CD
J Biol Chem 281 17400-17409 (2006)
PMID: 16624804

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115137 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein K2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 111 amino acids
Theoretical weight: 12.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q01167 (Residues: 255-353; Coverage: 15%)
Gene names: FOXK2, ILF, ILF1
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
INTERLEUKIN 2 PROMOTOR Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Homo sapiens
Expression system: Not provided
INTERLEUKIN 2 PROMOTOR Chain: D
Molecule details ›
Chain: D
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P6122
Unit cell:
a: 58.736Å b: 58.736Å c: 324.923Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.258
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

10 review citations

The evolution of Fox genes and their role in development and disease.
Hannenhalli et al. (2009)
9 more

16 mentions without citation

Toward a mechanistic understanding of DNA binding by forkhead transcription factors and its perturbation by pathogenic mutations.
Dai et al. (2021)
15 more