› 2c3x

X-ray diffraction
2.7Å resolution

Structure of iodinated CBM25 from Bacillus halodurans amylase in complex with maltotetraose

Released:
DOI: 10.2210/pdb2c3x/pdb
PDB entry 2c3x coloured by chain, front view.
PDB entry 2c3x coloured by chain, side view.
PDB entry 2c3x coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
A structural and functional analysis of alpha-glucan recognition by family 25 and 26 carbohydrate-binding modules reveals a conserved mode of starch recognition.
Boraston AB, Healey M, Klassen J, Ficko-Blean E, Lammerts van Bueren A, Law V
J Biol Chem 281 587-98 (2006)
PMID: 16230347

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-amylase G-6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 102 amino acids
Theoretical weight: 11.14 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KFR4 (Residues: 863-958; Coverage: 10%)
Gene name: BH0413
Sequence domains: Carbohydrate binding domain (family 25)
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 64.743Å b: 64.743Å c: 187.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.212 0.283
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The carbohydrate-binding module family 20--diversity, structure, and function.
Christiansen et al. (2009)
9 more

1 mention without citation

Solution structure of family 21 carbohydrate-binding module from Rhizopus oryzae glucoamylase.
Liu et al. (2007)