› 2c3w

X-ray diffraction
1.81Å resolution

Structure of CBM25 from Bacillus halodurans amylase in complex with maltotetraose

Released:
DOI: 10.2210/pdb2c3w/pdb
PDB entry 2c3w coloured by chain, front view.
PDB entry 2c3w coloured by chain, side view.
PDB entry 2c3w coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
A structural and functional analysis of alpha-glucan recognition by family 25 and 26 carbohydrate-binding modules reveals a conserved mode of starch recognition.
Boraston AB, Healey M, Klassen J, Ficko-Blean E, Lammerts van Bueren A, Law V
J Biol Chem 281 587-98 (2006)
PMID: 16230347

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Alpha-amylase G-6 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 102 amino acids
Theoretical weight: 11.14 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KFR4 (Residues: 863-958; Coverage: 10%)
Gene name: BH0413
Sequence domains: Carbohydrate binding domain (family 25)
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 47.533Å b: 94.553Å c: 64.868Å
α: 90° β: 104.65° γ: 90°
R-values:
R R work R free
0.161 0.158 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

The carbohydrate-binding module family 20--diversity, structure, and function.
Christiansen et al. (2009)
9 more

1 mention without citation

Hydrophilic aromatic residue and in silico structure for carbohydrate binding module.
Chou et al. (2011)