› 2c2n

X-ray diffraction
1.55Å resolution

Structure of human mitochondrial malonyltransferase

Released:
DOI: 10.2210/pdb2c2n/pdb
PDB entry 2c2n coloured by chain, front view.
PDB entry 2c2n coloured by chain, side view.
PDB entry 2c2n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for different specificities of acyltransferases associated with the human cytosolic and mitochondrial fatty acid synthases.
Bunkoczi G, Misquitta S, Wu X, Lee WH, Rojkova A, Kochan G, Kavanagh KL, Oppermann U, Smith S
Chem Biol 16 667-75 (2009)
PMID: 19549604

Function and Biology Details

Reaction catalysed: 
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malonyl-CoA-acyl carrier protein transacylase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 339 amino acids
Theoretical weight: 37.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IVS2 (Residues: 60-375; Coverage: 81%)
Gene names: MCAT, MT
Sequence domains: Acyl transferase domain
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand SO4 8 x SO4
Ligand AE4 1 x AE4
Ligand DXE 1 x DXE
Ligand CL 1 x CL
Ligand AE3 1 x AE3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 52.573Å b: 84.828Å c: 74.565Å
α: 90° β: 93.43° γ: 90°
R-values:
R R work R free
0.179 0.178 0.218
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

The metabolic serine hydrolases and their functions in mammalian physiology and disease.
Long et al. (2011)
2 more

8 mentions without citation

Blueprint for antimicrobial hit discovery targeting metabolic networks.
Shen et al. (2010)
7 more