› 2bwh

X-ray diffraction
1.9Å resolution

Laue Structure of a Short Lived State of L29W Myoglobin

Released:
DOI: 10.2210/pdb2bwh/pdb
PDB entry 2bwh coloured by chain, front view.
PDB entry 2bwh coloured by chain, side view.
PDB entry 2bwh coloured by chain, top view.
Source organism: Physeter catodon
Primary publication:
Ligand migration pathway and protein dynamics in myoglobin: a time-resolved crystallographic study on L29W MbCO.
Schmidt M, Nienhaus K, Pahl R, Krasselt A, Anderson S, Parak F, Nienhaus GU, Srajer V
Proc Natl Acad Sci U S A 102 11704-9 (2005)
PMID: 16085709

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myoglobin Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.31 KDa
Source organism: Physeter catodon
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02185 (Residues: 2-154; Coverage: 99%)
Gene name: MB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand CMO 1 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P6
Unit cell:
a: 91.87Å b: 91.87Å c: 46.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Ligand diffusion in globins: simulations versus experiment.
Elber R. (2010)
10 more

3 mentions without citation

Ligand migration pathway and protein dynamics in myoglobin: a time-resolved crystallographic study on L29W MbCO.
Schmidt et al. (2005)
2 more