› 2b94

X-ray diffraction
1.85Å resolution

Structural analysis of P knowlesi homolog of P falciparum PNP

Released:
DOI: 10.2210/pdb2b94/pdb
PDB entry 2b94 coloured by chain, front view.
PDB entry 2b94 coloured by chain, side view.
PDB entry 2b94 coloured by chain, top view.
Source organism: Plasmodium knowlesi
Entry authors: Robien MA, Bosch J, Hol WGJ, Structural Genomics of Pathogenic Protozoa Consortium (SGPP)

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside phosphorylase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 29.91 KDa
Source organism: Plasmodium knowlesi
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VWV7 (Residues: 1-245; Coverage: 100%)
Gene names: PKNOH_S07457800, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand THJ 2 x THJ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: R32
Unit cell:
a: 98.377Å b: 98.377Å c: 160.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.187 0.225
Expression system: Escherichia coli

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