› 2b2j

X-ray diffraction
1.85Å resolution

Ammonium Transporter Amt-1 from A. fulgidus (Xe)

Released:
DOI: 10.2210/pdb2b2j/pdb
PDB entry 2b2j coloured by chain, front view.
PDB entry 2b2j coloured by chain, side view.
PDB entry 2b2j coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.
Andrade SL, Dickmanns A, Ficner R, Einsle O
Proc Natl Acad Sci U S A 102 14994-9 (2005)
PMID: 16214888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter Amt1 Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 42.09 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29285 (Residues: 1-391; Coverage: 100%)
Gene names: AF_0977, amt1
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

1 bound ligand:
Ligand XE 15 x XE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R3
Unit cell:
a: 111.46Å b: 111.46Å c: 135.578Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.221
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Nitrogen regulation in bacteria and archaea.
Leigh et al. (2007)
27 more

2 mentions without citation

High-resolution structure prediction and the crystallographic phase problem.
Qian et al. (2007)
1 more