› 2anx

X-ray diffraction
1.04Å resolution

crystal structure of bacteriophage P22 lysozyme mutant L87M

Released:
DOI: 10.2210/pdb2anx/pdb
PDB entry 2anx coloured by chain, front view.
PDB entry 2anx coloured by chain, side view.
PDB entry 2anx coloured by chain, top view.
Source organism: Salmonella virus P22
Primary publication:
Extension to 2268 atoms of direct methods in the ab initio determination of the unknown structure of bacteriophage P22 lysozyme.
Mooers BH, Matthews BW
Acta Crystallogr D Biol Crystallogr 62 165-76 (2006)
PMID: 16421448

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140868 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B
Molecule details ›
Chains: A, B
Length: 146 amino acids
Theoretical weight: 16.18 KDa
Source organism: Salmonella virus P22
Expression system: Escherichia coli
UniProt:
  • Canonical: P09963 (Residues: 1-146; Coverage: 100%)
Gene name: 19
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

4 bound ligands:
Ligand SO4 6 x SO4
Ligand SM 3 x SM
Ligand IOD 8 x IOD
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 134.013Å b: 50.415Å c: 46.587Å
α: 90° β: 103.8° γ: 90°
R-values:
R R work R free
0.117 0.117 0.15
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacteriophage endolysins as novel antimicrobials.
Schmelcher et al. (2012)
3 more

5 mentions without citation

Cell Wall Hydrolases in Bacteria: Insight on the Diversity of Cell Wall Amidases, Glycosidases and Peptidases Toward Peptidoglycan.
Vermassen et al. (2019)
4 more