› 2amo

X-ray diffraction
2.6Å resolution

Loose Dimer of a Bacillus subtilis Nitric Oxide Synthase

Released:
DOI: 10.2210/pdb2amo/pdb
PDB entry 2amo coloured by chain, front view.
PDB entry 2amo coloured by chain, side view.
PDB entry 2amo coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of a loose dimer: an intermediate in nitric oxide synthase assembly.
Pant K, Crane BR
J Mol Biol 352 932-40 (2005)
PMID: 16126221

Function and Biology Details

Reaction catalysed: 
(1a) 2 L-arginine + 2 reduced flavodoxin + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 oxidized flavodoxin + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase oxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 41.48 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O34453 (Residues: 30-363; Coverage: 92%)
Gene names: BSU07630, nos, yflM
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2, NSLS BEAMLINE X26C
Spacegroup: P21212
Unit cell:
a: 81.314Å b: 93.211Å c: 118.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.274 0.29
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Bacterial nitric oxide synthases.
Crane et al. (2010)
9 more

1 mention without citation

Substrate-ligand interactions in Geobacillus stearothermophilus nitric oxide synthase.
Kabir et al. (2008)