› 2aky

X-ray diffraction
1.96Å resolution

HIGH-RESOLUTION STRUCTURES OF ADENYLATE KINASE FROM YEAST LIGATED WITH INHIBITOR AP5A, SHOWING THE PATHWAY OF PHOSPHORYL TRANSFER

Released:
DOI: 10.2210/pdb2aky/pdb
PDB entry 2aky coloured by chain, front view.
PDB entry 2aky coloured by chain, side view.
PDB entry 2aky coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
High-resolution structures of adenylate kinase from yeast ligated with inhibitor Ap5A, showing the pathway of phosphoryl transfer.
Abele U, Schulz GE
Protein Sci 4 1262-71 (1995)
PMID: 7670369

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139418 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chain: A
Molecule details ›
Chain: A
Length: 220 amino acids
Theoretical weight: 24.07 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07170 (Residues: 3-222; Coverage: 99%)
Gene names: ADK1, AKY, AKY1, AKY2, YD9934.11, YDR226W
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand AP5 1 x AP5
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 36Å b: 40.4Å c: 45.5Å
α: 110.7° β: 108.8° γ: 64.7°
R-values:
R R work R free
0.176 0.176 not available
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Sequence analysis of the AAA protein family.
Beyer A. (1997)
4 more

6 mentions without citation

Zipping and unzipping of adenylate kinase: atomistic insights into the ensemble of open<-->closed transitions.
Beckstein et al. (2009)
5 more