› 2akh

Electron Microscopy
14.9Å resolution

Normal mode-based flexible fitted coordinates of a non-translocating SecYEG protein-conducting channel into the cryo-EM map of a SecYEG-nascent chain-70S ribosome complex from E. coli

Released:
DOI: 10.2210/pdb2akh/pdb
PDB entry 2akh coloured by chain, front view.
PDB entry 2akh coloured by chain, side view.
PDB entry 2akh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the E. coli protein-conducting channel bound to a translating ribosome.
Mitra K, Schaffitzel C, Shaikh T, Tama F, Jenni S, Brooks CL, Ban N, Frank J
Nature 438 318-24 (2005)
PMID: 16292303
Related structures: EMD-1143

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142802 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein-export membrane protein SecG Chains: A, X
Molecule details ›
Chains: A, X
Length: 77 amino acids
Theoretical weight: 7.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG99 (Residues: 1-77; Coverage: 70%)
Gene names: JW3142, b3175, secG
Sequence domains: Preprotein translocase SecG subunit
Protein translocase subunit SecY Chains: B, Y
Molecule details ›
Chains: B, Y
Length: 400 amino acids
Theoretical weight: 43.96 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGA2 (Residues: 1-436; Coverage: 90%)
Gene names: JW3262, b3300, prlA, secY
Sequence domains: SecY
Protein translocase subunit SecE Chains: C, Z
Molecule details ›
Chains: C, Z
Length: 111 amino acids
Theoretical weight: 12.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AG96 (Residues: 17-127; Coverage: 87%)
Gene names: JW3944, b3981, prlG, secE
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 14.9Å
Relevant EMDB volumes: EMD-1143
Expression system: Escherichia coli

Quick links

Citations

50 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
49 more

2 mentions without citation

Structure of the E. coli protein-conducting channel bound to a translating ribosome.
Mitra et al. (2005)
1 more