› 2afp

Solution NMR

THE SOLUTION STRUCTURE OF TYPE II ANTIFREEZE PROTEIN REVEALS A NEW MEMBER OF THE LECTIN FAMILY

Released:
DOI: 10.2210/pdb2afp/pdb
PDB entry 2afp coloured by chain, ensemble of 5 models, front view.
PDB entry 2afp coloured by chain, ensemble of 5 models, side view.
PDB entry 2afp coloured by chain, ensemble of 5 models, top view.
Source organism: Hemitripterus americanus
Primary publication:
The solution structure of type II antifreeze protein reveals a new member of the lectin family.
Gronwald W, Loewen MC, Lix B, Daugulis AJ, Sönnichsen FD, Davies PL, Sykes BD
Biochemistry 37 4712-21 (1998)
PMID: 9537986

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138557 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type-2 ice-structuring protein Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14 KDa
Source organism: Hemitripterus americanus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P05140 (Residues: 35-163; Coverage: 88%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Komagataella pastoris

Quick links

Citations

20 review citations

Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
Cereghino et al. (2000)
19 more

7 mentions without citation

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture.
Eskandari et al. (2020)
6 more