› 2a7b

X-ray diffraction
1.65Å resolution

On the Routine Use of Soft X-Rays in Macromolecular Crystallography, Part III- The Optimal Data Collection Wavelength

Released:
DOI: 10.2210/pdb2a7b/pdb
PDB entry 2a7b coloured by chain, front view.
PDB entry 2a7b coloured by chain, side view.
PDB entry 2a7b coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
On the routine use of soft X-rays in macromolecular crystallography. Part III. The optimal data-collection wavelength.
Mueller-Dieckmann C, Panjikar S, Tucker PA, Weiss MS
Acta Crystallogr D Biol Crystallogr 61 1263-72 (2005)
PMID: 16131760

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149822 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AP-1 complex subunit gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.42 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P22892 (Residues: 704-822; Coverage: 15%)
Gene names: Adtg, Ap1g1, Clapg1
Sequence domains: Adaptin C-terminal domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand XE 2 x XE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 33.43Å b: 53.76Å c: 66.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

SAD phasing: History, current impact and future opportunities.
Rose et al. (2016)
1 more

2 mentions without citation

Computational design of a symmetric homodimer using β-strand assembly.
Stranges et al. (2011)
1 more