› 2a71

X-ray diffraction
2.7Å resolution

Crystal structure of Emp47p carbohydrate recognition domain (CRD), orthorhombic crystal form

Released:
DOI: 10.2210/pdb2a71/pdb
PDB entry 2a71 coloured by chain, front view.
PDB entry 2a71 coloured by chain, side view.
PDB entry 2a71 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of the carbohydrate recognition domain of Ca2+-independent cargo receptors Emp46p and Emp47p.
Satoh T, Sato K, Kanoh A, Yamashita K, Yamada Y, Igarashi N, Kato R, Nakano A, Wakatsuki S
J Biol Chem 281 10410-9 (2006)
PMID: 16439369

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein EMP47 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 222 amino acids
Theoretical weight: 24.82 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P43555 (Residues: 34-255; Coverage: 53%)
Gene names: EMP47, YFL048C
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 39.59Å b: 129.62Å c: 170.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Receptor-mediated protein transport in the early secretory pathway.
Baines et al. (2007)
2 more