› 2a5c

X-ray diffraction
2.5Å resolution

Structure of Avidin in complex with the ligand 8-oxodeoxyadenosine

Released:
DOI: 10.2210/pdb2a5c/pdb
PDB entry 2a5c coloured by chain, front view.
PDB entry 2a5c coloured by chain, side view.
PDB entry 2a5c coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Recognition of oxidatively modified bases within the biotin-binding site of avidin.
Conners R, Hooley E, Clarke AR, Thomas S, Brady RL
J Mol Biol 357 263-74 (2006)
PMID: 16413579

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Avidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 13.71 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P02701 (Residues: 25-147; Coverage: 96%)
Gene name: AVD
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand NAG 2 x NAG
Ligand 8DA 2 x 8DA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P21212
Unit cell:
a: 70.584Å b: 79.052Å c: 42.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.221 0.308

Quick links

Citations

1 review citation

Oxidatively generated damage to cellular DNA by UVB and UVA radiation.
Cadet et al. (2015)
9 other articles

2 mentions without citation

Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.
Bloom et al. (2007)
1 more