› 2a53

X-ray diffraction
1.45Å resolution

fluorescent protein asFP595, A143S, off-state

Released:
DOI: 10.2210/pdb2a53/pdb
PDB entry 2a53 coloured by chain, front view.
PDB entry 2a53 coloured by chain, side view.
PDB entry 2a53 coloured by chain, top view.
Source organism: Anemonia sulcata
Primary publication:
Structure and mechanism of the reversible photoswitch of a fluorescent protein.
Andresen M, Wahl MC, Stiel AC, Gräter F, Schäfer LV, Trowitzsch S, Weber G, Eggeling C, Grubmüller H, Hell SW, Jakobs S
Proc Natl Acad Sci U S A 102 13070-4 (2005)
PMID: 16135569

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190440 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GFP-like non-fluorescent chromoprotein FP595 chain 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 73 amino acids
Theoretical weight: 8.07 KDa
Source organism: Anemonia sulcata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZ28 (Residues: 2-62; Coverage: 26%)
Structure domains: Pantoate--beta-alanine Ligase; Chain: A,domain 2
GFP-like non-fluorescent chromoprotein FP595 chain 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 168 amino acids
Theoretical weight: 19.17 KDa
Source organism: Anemonia sulcata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZ28 (Residues: 63-232; Coverage: 73%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2221
Unit cell:
a: 75.996Å b: 126.259Å c: 93.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.173 0.199
Expression system: Escherichia coli

Quick links

Citations

36 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
35 more

1 mention without citation

Structure and mechanism of the reversible photoswitch of a fluorescent protein.
Andresen et al. (2005)