› 1yir

X-ray diffraction
2.1Å resolution

Crystal Structure of a Nicotinate Phosphoribosyltransferase

Released:
DOI: 10.2210/pdb1yir/pdb
PDB entry 1yir coloured by chain, front view.
PDB entry 1yir coloured by chain, side view.
PDB entry 1yir coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Entry authors: Seetharaman J, Swaminathan S, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H(2)O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinate phosphoribosyltransferase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 408 amino acids
Theoretical weight: 46.96 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HW26 (Residues: 2-398; Coverage: 100%)
Gene names: PA4376, pncB2
Sequence domains:
Structure domains: nicotinate phosphoribosyltransferase

Ligands and Environments

1 bound ligand:
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 222.599Å b: 113.355Å c: 93.231Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.213 0.213 0.247
Expression system: Escherichia coli

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