› 1yfm

X-ray diffraction
2.6Å resolution

RECOMBINANT YEAST FUMARASE

Released:
DOI: 10.2210/pdb1yfm/pdb
PDB entry 1yfm coloured by chain, front view.
PDB entry 1yfm coloured by chain, side view.
PDB entry 1yfm coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structures of native and recombinant yeast fumarase.
Weaver T, Lees M, Zaitsev V, Zaitseva I, Duke E, Lindley P, McSweeny S, Svensson A, Keruchenko J, Keruchenko I, Gladilin K, Banaszak L
J Mol Biol 280 431-42 (1998)
PMID: 9665847

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 53.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P08417 (Residues: 1-488; Coverage: 100%)
Gene names: FUM1, YPL262W
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P42212
Unit cell:
a: 95.19Å b: 95.19Å c: 100.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.315
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Targeting of proteins to mitochondria.
Lithgow T. (2000)
3 more

5 mentions without citation

Structural basis of fumarate hydratase deficiency.
Picaud et al. (2011)
4 more