› 1q0a

X-ray diffraction
2Å resolution

Crystal structure of the Zn(II) form of E. coli ZntR, a zinc-sensing transcriptional regulator (space group C222)

Released:
DOI: 10.2210/pdb1q0a/pdb
PDB entry 1q0a coloured by chain, front view.
PDB entry 1q0a coloured by chain, side view.
PDB entry 1q0a coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR.
Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragón A
Science 301 1383-7 (2003)
PMID: 12958362

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142295 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator ZntR Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 11.11 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACS5 (Residues: 43-141; Coverage: 70%)
Gene names: JW3254, b3292, yhdM, zntR
Sequence domains: MerR, DNA binding
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C222
Unit cell:
a: 84.666Å b: 170.707Å c: 42.208Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

76 review citations

Antimicrobial activity of metals: mechanisms, molecular targets and applications.
Lemire et al. (2013)
75 more

1 mention without citation

Identification of Position-Specific Correlations between DNA-Binding Domains and Their Binding Sites. Application to the MerR Family of Transcription Factors.
Korostelev et al. (2016)