› 1nff

X-ray diffraction
1.8Å resolution

Crystal structure of Rv2002 gene product from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1nff/pdb
PDB entry 1nff coloured by chain, front view.
PDB entry 1nff coloured by chain, side view.
PDB entry 1nff coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis.
Yang JK, Park MS, Waldo GS, Suh SW
Proc Natl Acad Sci U S A 100 455-60 (2003)
PMID: 12524453

Function and Biology Details

Reaction catalysed: 
Androstan-3-alpha,17-beta-diol + NAD(+) = 17-beta-hydroxyandrostan-3-one + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 27.1 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGT1 (Residues: 1-260; Coverage: 100%)
Gene names: MTCY39.16c, Rv2002, fabG3
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P3221
Unit cell:
a: 70.377Å b: 70.377Å c: 148.934Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.164 0.199
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Laboratory-directed protein evolution.
Yuan et al. (2005)
3 more

2 mentions without citation

Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis.
Yang et al. (2003)
1 more