1ipe

X-ray diffraction
2.5Å resolution

TROPINONE REDUCTASE-II COMPLEXED WITH NADPH

Released:

Function and Biology Details

Reaction catalysed:
Pseudotropine + NADP(+) = tropinone + NADPH
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-156139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tropinone reductase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 259 amino acids
Theoretical weight: 28.21 KDa
Source organism: Datura stramonium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50163 (Residues: 2-260; Coverage: 100%)
Gene name: TR2
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P6122
Unit cell:
a: 88.6Å b: 88.6Å c: 338.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.195 0.268
Expression system: Escherichia coli BL21(DE3)