› 1fx8

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE E. COLI GLYCEROL FACILITATOR (GLPF) WITH SUBSTRATE GLYCEROL

Released:
DOI: 10.2210/pdb1fx8/pdb
PDB entry 1fx8 coloured by chain, front view.
PDB entry 1fx8 coloured by chain, side view.
PDB entry 1fx8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of a glycerol-conducting channel and the basis for its selectivity.
Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, Krucinski J, Stroud RM
Science 290 481-6 (2000)
PMID: 11039922

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol uptake facilitator protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 29.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AER0 (Residues: 1-281; Coverage: 100%)
Gene names: JW3898, b3927, glpF
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

2 bound ligands:
Ligand BOG 3 x BOG
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: I422
Unit cell:
a: 96.76Å b: 96.76Å c: 184.322Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.223
Expression system: Escherichia coli

Quick links

Citations

143 review citations

Osmotic stress signaling and osmoadaptation in yeasts.
Hohmann S. (2002)
142 more

94 mentions without citation

Aquaporins: important but elusive drug targets.
Verkman et al. (2014)
93 more