› 1fvg

X-ray diffraction
1.6Å resolution

CRYSTAL STRUCTURE OF BOVINE PEPTIDE METHIONINE SULFOXIDE REDUCTASE

Released:
DOI: 10.2210/pdb1fvg/pdb
PDB entry 1fvg coloured by chain, front view.
PDB entry 1fvg coloured by chain, side view.
PDB entry 1fvg coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure and mechanism of peptide methionine sulfoxide reductase, an "anti-oxidation" enzyme.
Lowther WT, Brot N, Weissbach H, Matthews BW
Biochemistry 39 13307-12 (2000)
PMID: 11063566

Function and Biology Details

Reaction catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156950 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitochondrial peptide methionine sulfoxide reductase Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.39 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54149 (Residues: 21-219; Coverage: 85%)
Gene name: MSRA
Sequence domains: Peptide methionine sulfoxide reductase
Structure domains: Peptide methionine sulphoxide reductase MsrA

Ligands and Environments

1 bound ligand:
Ligand DTT 1 x DTT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 45.757Å b: 76.883Å c: 56.745Å
α: 90° β: 109.21° γ: 90°
R-values:
R R work R free
0.182 0.182 0.242
Expression system: Escherichia coli

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Citations

19 review citations

Oxidative stress and protein aggregation during biological aging.
Squier TC. (2001)
18 more

3 mentions without citation

Identifying unexpected therapeutic targets via chemical-protein interactome.
Yang et al. (2010)
2 more