› 1zyl

X-ray diffraction
2.8Å resolution

Crystal Structure of Hypothetical Protein YihE from Escherichia coli

Released:
DOI: 10.2210/pdb1zyl/pdb
PDB entry 1zyl coloured by chain, front view.
PDB entry 1zyl coloured by chain, side view.
PDB entry 1zyl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a novel prokaryotic Ser/Thr kinase and its implication in the Cpx stress response pathway.
Zheng J, He C, Singh VK, Martin NL, Jia Z
Mol Microbiol 63 1360-71 (2007)
PMID: 17302814

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142890 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stress response kinase A Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 38.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0C0K3 (Residues: 1-328; Coverage: 100%)
Gene names: JW3831, b3859, rdoA, srkA, yihE
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 90.974Å b: 90.974Å c: 110.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Eukaryote-like serine/threonine kinases and phosphatases in bacteria.
Pereira et al. (2011)
4 more

6 mentions without citation

A novel protein kinase-like domain in a selenoprotein, widespread in the tree of life.
Dudkiewicz et al. (2012)
5 more